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We applied zooarchaeology by mass spectrometry (Zoo MS) to find additional hominin remains.

We identified one bone that is Neanderthal, based on its mitochondrial DNA, and dated it directly to 46,200 ± 1,500 B. We also attempted to date six early Upper Paleolithic bone points from stratigraphic units G.

The data show that all the Neanderthal remains are from a much earlier period ( Previous dating of the Vi-207 and Vi-208 Neanderthal remains from Vindija Cave (Croatia) led to the suggestion that Neanderthals survived there as recently as 28,000–29,000 B. Subsequent dating yielded older dates, interpreted as ages of at least ∼32,500 B. We have redated these same specimens using an approach based on the extraction of the amino acid hydroxyproline, using preparative high-performance liquid chromatography (Prep-HPLC).

This method is more efficient in eliminating modern contamination in the bone collagen. P., suggesting the Vindija Neanderthals did not live more recently than others across Europe, and probably predate the arrival of anatomically modern humans in Eastern Europe.

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